Study on Distribution of <i>Acinetobacter baumannii</i> Complex in Dental Hospital Using Multiplex PCR

Purpose: In recent years, multidrug-resistant Acinetobacter baumannii has appeared and caused outbreaks of hospital infections all over the world. Close monitoring this pathogen other A. baumanii complex species is considered critical importance to public health organizations. The reliable identification method able distinguish from genetically close needed, because these are unable be differentiated by phenotypic or biochemical methods. purpose present study was design species-specific primers in order identify detect species, lwoffii which frequently detected human specimens, investigate distribution organisms dental using a multiplex PCR. Methods: Polymerase chain reaction (PCR) were designed based on partial sequences 16S ribosomal RNA (16S rRNA) gene DNA gyrase subunit B (gyrB) each species. Swab samples collected ten spittoon units hospital, investigated Results: These clearly. calcoaceticus at 20.0% 10.0% swab samples, respectively. On hand, nosocomialis, lowffii, pittii no sample. Conclusion: Our developed one-step PCR accurate, specific, cost-effective, time-saving, worked without requiring extraction.